Enhanced Endothelial Cell Growth on NaOH-Treated Electrospun PET Coated with Fibrin

Nur Syazana Rashidi, Irza Sukmana, Mohammed Rafiq Abdul Kadir — Mon, 30 Dec 2024 00:00:00 +0700

Polyethylene terephthalate (PET) hydrophobic nature may lead to problems, particularly protein absorption, when used for biomedical application. Thus, NaOH surface treatment was introduced towards electrospun PET fiber, to improve the hydrophilicity of the fiber. This investigation involved treating electrospun PET fiber with sodium hydroxide (NaOH) at 65°C for one hour. Fibrin gel was subsequently applied to NaOH-treated samples and uncoated PET for comparative analysis prior to the seeding of human umbilical vein endothelial cells (HUVECs) for durations of 2, 4, and 6 hours. The NaOH treatment led to increased hydrophilicity attributed to the presence of carboxyl and hydroxyl groups, as verified by EDS mapping data. Following the application of fibrin gel to NaOH-treated PET, in vitro culture with HUVEC cells demonstrated enhanced cellular growing up to 6 hours of culture. The PET fibers treated with NaOH and coated with fibrin were demonstrated to attract HUVEC cells and enhance endothelialization.

In-Vitro NaOH Surface-Treated and Fibrin-Coated Electrospun Polyethylene Terephthalate Scaffolds for Small Diameter Vascular Graft

Nur Syazana Rashidi, Irza Sukmana, Mohammed Rafiq Abdul Kadir — Mon, 30 Dec 2024 00:00:00 +0700

The surface inertness of polyethylene terephthalate (PET) gives disadvantages including noncompliance, thrombogenicity, intimal hyperplasia, aneurysms, calcium deposition and infection also limited growth for small diameter vascular graft application thus limits their efficacy. To overcome this problem, electrospun PET fiber was treated with sodium hydroxide (NaOH) at 65^oC for 1-hour. Fibrin gel was then coated onto NaOH treated samples and bare PET for comparison before seeding human umbilical vein endothelial cells (HUVECs) for 1, 7 and 14 days. The NaOH treatment resulted in reduced fibre diameter, enhanced hydrophilicity due to the existence of carboxyl and hydroxyl groups confirmed by FTIR results and contact angle measurements showed that the hydrophilicity of NaOH treated samples improved from 123° to 86°. After fibrin gel was coated on PET treated NaOH, in vitro tests showed increased cell adhesion and proliferation after 14 days of culture also formation of collagen after 21 days of culture. The PET treated with NaOH fibers coated with fibrin was proven attract HUVEC cells and promote endothelialization.

Semarak Current Biomedical Technology Research Journal

Enhanced Endothelial Cell Growth on NaOH-Treated Electrospun PET Coated with Fibrin

In-Vitro NaOH Surface-Treated and Fibrin-Coated Electrospun Polyethylene Terephthalate Scaffolds for Small Diameter Vascular Graft