Synergism effect of Murraya koneigii  and Piper betle leaves extracts on antibacterial activity against Gram Positive bacteria

Putri Shareen Rosman; Syarmilla Ezza Ismail; Mohd Khairul Bahri; Nur Syazdiyana Mohd Azmali; Suat Hian Tan

doi:10.37934/araset.28.2.311317

Authors

Putri Shareen Rosman Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, Tapah Road, Perak, Malaysia
Syarmilla Ezza Ismail Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang Kuantan, Pahang, Malaysia
Mohd Khairul Bahri Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, Tapah Road, Perak, Malaysia
Nur Syazdiyana Mohd Azmali Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, Tapah Road, Perak, Malaysia
Suat Hian Tan Department of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, Tapah Road, Perak, Malaysia

DOI:

https://doi.org/10.37934/araset.28.2.311317

Keywords:

Antagonism, Antibacterial activity, P.betle, M.koneigii

Abstract

Antimicrobial resistance has become the major threat to the health worldwide. It has lead to the ineffective impact of the ability of the substance or antibiotics to kill the bacteria. Therefore,there is a need to find a new alternative to overcome the problem by using natural products. In this study Piper betle (P. betle) and Murraya koenigii (M. koenigii) leaf extracts were used to measure their synergism effect towards antimicrobial treatments. Previous study has shown that both plants has shown their ability in inhibiting the growth of bacteria separately. Three different concentration ratios of the synergised leaf extracts were used with 1:1(P.betle:M. koenigii) ratios, 2:1 (P.betle:M. koenigii) ratios and 1:2 (P.betle:M.koenigii) ratios.The synergised leaf extracts was at the highest inhibition zone with combination of 2:1 ratio of P.betle and M. koenigii with 22.6mm. However, it does not have the greater inhibition ability as compared to the P.betle leaf extract with 28.1mm . Therefore, from the study, antagonism effect was discovered when both plant extracts were combined.