Antioxidant Assay of Alstonia Angustifolia Ethanolic Leaf Extract

Authors

  • Nurhidayah Ab Rahim Medical Laboratory Technology, Faculty of Health Science, University Technology of MARA (Bertam Campus), 13200 Pulau Pinang, Malaysia
  • Noorzafiza Zakaria Medical Laboratory Technology, Faculty of Health Science, University Technology of MARA (Bertam Campus), 13200 Pulau Pinang, Malaysia
  • Syarifah Masyitah Habib Dzulkarnain Medical Laboratory Technology, Faculty of Health Science, University Technology of MARA (Bertam Campus), 13200 Pulau Pinang, Malaysia
  • Nazar Mohd Zabadi Mohd Azahar Medical Laboratory Technology, Faculty of Health Science, University Technology of MARA (Bertam Campus), 13200 Pulau Pinang, Malaysia
  • Mahmood Ameen Abdullah Biomedical Science,University of Malaya, 50603 Kuala Lumpur, Malaysia

Keywords:

Alstonia angustifolia, antioxidant assay, DPPH, FRAP, Hydrogen peroxide scavenging assay

Abstract

In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaveshas a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 μM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 μM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 μM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 μg/ml and 39.60 μg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 μg/ml compared to standard ascorbic acid 466.56 μg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extracthas a good origin of natural antioxidants and mightbe beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.

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Published

2018-02-15

How to Cite

Nurhidayah Ab Rahim, Noorzafiza Zakaria, Syarifah Masyitah Habib Dzulkarnain, Nazar Mohd Zabadi Mohd Azahar, & Mahmood Ameen Abdullah. (2018). Antioxidant Assay of Alstonia Angustifolia Ethanolic Leaf Extract. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 42(1), 80–86. Retrieved from https://semarakilmu.com.my/journals/index.php/fluid_mechanics_thermal_sciences/article/view/2656

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