Antioxidant Assay of Alstonia Angustifolia Ethanolic Leaf Extract
Keywords:
Alstonia angustifolia, antioxidant assay, DPPH, FRAP, Hydrogen peroxide scavenging assayAbstract
In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaveshas a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 μM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 μM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 μM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 μg/ml and 39.60 μg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 μg/ml compared to standard ascorbic acid 466.56 μg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extracthas a good origin of natural antioxidants and mightbe beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.