Microwave-Assisted Green Synthesis of Ag Nanoparticles using Leaves of Melia Dubia(Neem) and its Antibacterial Activities

Mustafa Mudhafar; Ismail Zainol; Che Nor Aiza Jaafar; H. A. Alsailawi; Alhussein Arkan Majhool

Authors

Mustafa Mudhafar Department of Chemistry, Faculty of Science and Mathematics, Universiti PendidikanSultan Idris, Proton City, 35900 Tanjung Malim, Perak, Malaysia
Ismail Zainol Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Proton City, 35900 Tanjung Malim, Perak, Malaysia
Che Nor Aiza Jaafar Department of Mechanical and Manufacturing, Faculty of Engineering, Universiti Putra Malaysia, 43000 Serdang, Selangor, Malaysia
H. A. Alsailawi Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Proton City, 35900 Tanjung Malim, Perak, Malaysia
Alhussein Arkan Majhool Department of Chemistry, Faculty of Science and Mathematics, Universiti PendidikanSultan Idris, Proton City, 35900 Tanjung Malim, Perak, Malaysia

Keywords:

Microwave-assisted, AgNPs, Melia dubia (neem), antibacterial

Abstract

The biosynthesis methods of nanosilver are of interest due to its low cost and environmental friendliness compared with chemical and physical methods. This study will conduct a green synthesis of nanosilver by decreasing the AgNO3using leaf extract with the presence of stabilized microwave irradiation and collagen. Melia dubia leaves, and hydrolyzed fish scale collagen were utilized as reducing and stabilizing agents, respectively. Ag nanoparticles (AgNPs) weresynthesized and characterized via Uv-Vis spectroscopy, scanning electron microscope (SEM) that attached with energy-dispersive X-ray, X-ray diffraction (XRD), and antibacterial activities. Results showed that the spherical shape of AgNPs was formed with particle size ranging from 72 nm and 100 nm. UV–vis analysis revealed that the absorbance peak was observed at 446 nm, which corresponded to AgNPs. XRD analysis confirmed the natural crystalline of AgNPs with a particle size of 84.8 nm. The antimicrobial analysis was conducted using Staphylococcus aureus and Escherichia coli as Gram-positive and -negative bacteria, respectively. Results showed the inhibition zone against both bacteria, which indicated excellent antimicrobial properties of the samples.