Protein Profiles of Red Bigeye Flesh (Priacanthus Macracanthus)

Authors

  • Nor Salasiah Mohamed MARDI Kuala Terengganu, KM 10 Jalan Kelantan, 21200 Kuala Terengganu, Terengganu, Malaysia
  • Amiza Mat Amin Faculty of Fisheries and Food Science, University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • Dasi Ong Analytical Biochemistry Research Centre, University Sains Malaysia, 11800 Bayan Lepas, Pulau Pinang, Malaysia
  • Mohd Nazri Ismail Analytical Biochemistry Research Centre, University Sains Malaysia, 11800 Bayan Lepas, Pulau Pinang, Malaysia
  • Fisal Ahmad Food Security Research Cluster, University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

DOI:

https://doi.org/10.37934/aram.116.1.156168

Keywords:

Red bigeye, peptide, post-translational modifications, protein functional group

Abstract

Red bigeye fish with less than 15 cm length are categorized as low-value fish. The aim of this study was to determine the protein profiles of red bigeye flesh. Red bigeye flesh was analyzed for proximate analysis, LC-QTOF-MS analysis and Fourier transform infrared FTIR. It was found that red bigeye flesh contained 17.21% crude protein, 1.82% crude fat, 1.34% ash content and 3.42% carbohydrate. LC-MS/MS analysis revealed 83 unique proteins, including troponin 33, myosin 23, actin 19, tropomyosin 6, and parvalbumin 2. Regulatory proteins gave the largest portion of 51%, followed by enzymes at 28.92%, and unreviewed proteins at 20.48%. Red bigeye flesh proteins exhibited higher levels of post-translational modifications (PTMs) mainly oxidation and mutation. FTIR spectra analysis of red bigeye flesh protein exhibiting symmetrical bending stretches within amide regions A, B, I, II, III, and VII. This study shows that red bigeye flesh proteins is a promising source of functional food.

Author Biographies

Nor Salasiah Mohamed, MARDI Kuala Terengganu, KM 10 Jalan Kelantan, 21200 Kuala Terengganu, Terengganu, Malaysia

msalasiah@mardi.gov.my

Amiza Mat Amin, Faculty of Fisheries and Food Science, University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

ama@umt.edu.my

Dasi Ong, Analytical Biochemistry Research Centre, University Sains Malaysia, 11800 Bayan Lepas, Pulau Pinang, Malaysia

ong.dasi3@gmail.com

Mohd Nazri Ismail, Analytical Biochemistry Research Centre, University Sains Malaysia, 11800 Bayan Lepas, Pulau Pinang, Malaysia

mdnazri@usm.my

Fisal Ahmad, Food Security Research Cluster, University Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

fisal@umt.edu.my

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Published

2024-04-30

How to Cite

Nor Salasiah Mohamed, Amiza Mat Amin, Dasi Ong, Mohd Nazri Ismail, & Fisal Ahmad. (2024). Protein Profiles of Red Bigeye Flesh (Priacanthus Macracanthus) . Journal of Advanced Research in Applied Mechanics, 116(1), 156–168. https://doi.org/10.37934/aram.116.1.156168

Issue

Vol. 116 No. 1: April (2024)

Section

Articles