Optimization of Process Parameters of Immobilized Escherichia Coli for Cyclodextrin Production

Authors

  • Nur Nadia Mohd Zakaria Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Rohaida Che Man Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Siti Zubaidah Sulaiman Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Siti Kholijah Abdul Mudalip Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
  • Nor Hasmaliana Abdul Manas Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia
  • Laura Navika Yamani Department of Epidemiology, Biostatistics, Population Studies and Health Promotion, Faculty of Public Health, Universitas Airlangga, Campus C. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Kec. Mulyorejo Surabaya, Jawa Timur 60115, Indonesia

DOI:

https://doi.org/10.37934/aram.107.1.110

Keywords:

Cell immobilization, biocatalyst, Cyclodextrin, process parameter, Escherichia coli

Abstract

The growing interest toward cylclodectrin (CD) application in industries is due to its unique structure that able to form inclusion complexes with substances. However, the problems arise during the CD synthesis using the commercial enzyme (cyclodextrin glucanotransferase) are low production rate and the process is expensive. Hence, cell immobilization system was used. The objective of this study is to optimize the process parameters using Response Surface Methodology to increase the CD production of the immobilized cell. High CD production was achieved after the optimization process. The reusability of immobilized cell also can be reuse up to six cycles using the optimized parameters. The findings propose that optimization process increased the CD production and made it more valuable for industrial application.

Author Biographies

Nur Nadia Mohd Zakaria , Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia

nadiazakaria1201@gmail.com

Rohaida Che Man, Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia

rohaida@ump.edu.my

Siti Zubaidah Sulaiman, Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia

szubaidah@ump.edu.my

Siti Kholijah Abdul Mudalip , Faculty of Chemical and Process Engineering Technology, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia

kholijah@ump.edu.my

Nor Hasmaliana Abdul Manas , Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

hasmaliana@utm.my

Laura Navika Yamani, Department of Epidemiology, Biostatistics, Population Studies and Health Promotion, Faculty of Public Health, Universitas Airlangga, Campus C. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Kec. Mulyorejo Surabaya, Jawa Timur 60115, Indonesia

Laura Navika Yamani laura.navika@fkm.unair.ac.id

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Published

2023-09-01

How to Cite

Nur Nadia Mohd Zakaria, Rohaida Che Man, Siti Zubaidah Sulaiman, Siti Kholijah Abdul Mudalip, Nor Hasmaliana Abdul Manas, & Laura Navika Yamani. (2023). Optimization of Process Parameters of Immobilized Escherichia Coli for Cyclodextrin Production. Journal of Advanced Research in Applied Mechanics, 107(1), 1–10. https://doi.org/10.37934/aram.107.1.110

Issue

Vol. 107 No. 1: July (2023)

Section

Articles